Question: Why Do We Use Normal Saline In Microbiology?

What is the indication of normal saline?

The following are primary indications for the use of normal saline infusion that have been approved by the FDA: Extracellular fluid replacement (e.g., dehydration, hypovolemia, hemorrhage, sepsis) Treatment of metabolic alkalosis in the presence of fluid loss.

Mild sodium depletion..

What is the purpose of the saline solution?

Saline, also known as saline solution, is a mixture of sodium chloride in water and has a number of uses in medicine. Applied to the affected area it is used to clean wounds, help remove contact lenses, and help with dry eyes.

Why do we use saline for serial dilutions?

Saline solution is used for the preparation of culture suspension or serial dilution in microbiology. … The osmotic pressure develop on the bacterial cell which can damage the bacterial cells during serial dilution. That’s why serial dilution is prepared in 0.9% saline solution.

Can bacteria grow in normal saline?

Conclusions: No bacterial growth was noted in any of the 257 samples collected. Normal saline and dextrose 5% in water do not support bacterial growth 24 hours after their preparation using standard sterile techniques in the perioperative space.

How do you use normal saline?

Put one cup of water and ½ teaspoon of salt into the pot. Put the lid on.Boil for 15 minutes with the lid on (set a timer).Set the pan aside until cooled to a room temperature.Carefully pour the salt and water (normal saline) from the pan into the jar or bottle and put the lid on.

Why is 0.9 saline used?

0.9% Normal Saline (NS, 0.9NaCl, or NSS) Normal saline infusion is used for extracellular fluid replacement (e.g., dehydration, hypovolemia, hemorrhage, sepsis), treatment of metabolic alkalosis in the presence of fluid loss, and for mild sodium depletion.